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---DNA extraction
---DNA quantitation
---STR genotyping
---MPS STR analysis
---DNA methylation
------anlaysis
---Mitochondrial
------DNA anlaysis
---ABO genotyping
---PCR product
------purification

DNA extraction

- Blood, Buccal Swabs, or Bodyfluid using the QIAamp® DNA Mini Kit
- Hair or Casework sample using the QIAamp® DNA Investigator Kit
- Skeletal remains (bone and tooth)

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DNA quantitation

- Using the NanoDrop 1000 Spectrophotometer
- Using the Quantifiler™ Duo DNA Qunatification Kit
- Using the Quantifiler® HP and Trio DNA Quantification Kits

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STR genotyping

- Autosomal STRs
--------PowerPlex® Fusion System
--------GlobalFiler™ PCR Amplification Kit
--------Kplex-17 System (Autosomal STRs compatible with Identifiler loci)
--------Euplex-13 System (Autosomal STRs incorporated for expanded CODIS system)

- X-chromosomal STRs
--------Mentype® Argus X-8 PCR Amplification Kit
--------Multiplex III (DXS10146, DXS10103, DXS10148, DXS10079)

- Y-chromosomal STRs
--------PowerPlex® Y23 System
--------Kplex-Y18 System (Y-STRs compatible with Yfiler loci)
--------Euplex-Y17 System (Y-STRs incorporated for PowerPlex Y23 and RM-Y system)

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MPS STR analysis

- Referenec sample
--------Autosomal STR protocol and Config file for STRait Razor v3.0
--------Y-STR protocol and Config file for STRait Razor v3.0

- Degraded DNA
--------Autosomal STR protocol and Config file for STRait Razor v3.0
--------Y-STR protocol and Config file for STRait Razor v3.0

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DNA methylation analysis

- Body fluid identification
--------SNaPshot multiplex for 7 CpGs (Semen, blood, vaginal fluid, saliva)
--------SNaPshot multiplex for 9 CpGs (Semen, blood, vaginal fluid, saliva, menstrual blood)

- Age prediction
--------Saliva : SNaPshot multiplex and Age calculator
--------Semen : SNaPshot multiplex and Age calculator

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Mitochondrial DNA analysis

- Control region sequence
--------Multiplex PCR set for degraded DNA
--------Monoplex PCR sets for degraded DNA
----------------Midi-primer set
----------------Mini-primer set
--------Reference sample

- Coding region SNPs for East Asian mtDNA haplogroup

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ABO genotyping

- Simple ABO blood typing by allele specific PCR
- ABO blood typing for degraded DNA

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PCR product purification

- Using the QIAquick® PCR Purification Kit
- Using the ExoSAP-IT® for PCR Product Cleanup

 

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