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 DNA extraction
- Blood, Buccal Swabs, or Bodyfluid using the QIAamp® DNA Mini Kit
- Hair or Tissue using the Tissue and Hair Extraction Kit and the DNA IQ™ System
- Skeletal remains
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DNA quantitation
- Using the TBS-380 Mini-Fluorometer
- Using the NanoDrop 1000 Spectrophotometer
- Using the Quantifiler™ Kits
- Using the Quantifiler™ Duo DNA Qunatification Kit
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Mitochondrial DNA analysis
- Control region sequence
--------Skeletal remains
----------------Midi-primer set
----------------Mini-primer set
--------Blood or Buccal swab samples
- Length heteroplasmy
--------HV1 and HV2
--------Modified primer set for HV2 with reduced stutter
--------CA dinucleotide repeats
- Coding region SNPs for East Asian mtDNA haplogroup
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STR genotyping
- Autosomal STRs
--------AmpFlSTR® Identifiler® PCR Amplification Kit
--------PowerPlex® 16 System
- X-chromosoaml STRs
--------Multiplex I (GATA172D05, HPRTB, DXS8377, DXS101, HumARA)
--------Multiplex II (DXS7424, DXS9898, DXS6809, DXS10011)
--------Mentype® Argus X-8 PCR Amplification Kit
--------Multiplex III (DXS10146, DXS10103, DXS10148, DXS10079)
- Y-chromosoaml STRs
--------Multiplex I (Minimal haplotype STR loci)
--------Multiplex II (DYS385, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, GATA H4.1)
--------Multiplex III (DYS385, DYS388, DYS446, DYS447, DYS449, DYS464)
--------AmpFlSTR® Yfiler™ PCR Amplification Kit
--------PowerPlex® Y System
- Mini-STRs
--------AmpFlSTR® MiniFiler™ PCR Amplification Kit
--------Miniplex NC01+ (D10S1248, D14S1434, D22S1045, TPOX)
--------Y-miniplex I (DYS19, DYS389, DYS392, DYS437, DYS439, DYS448, DYS449, DYS456, DYS458,
----------------------- DYS635)
--------Y-miniplex II (DYS385, DYS388, DYS390, DYS391, DYS393, DYS438, DYS446, DYS447, DYS635,
----------------------- GATA H4.1)
--------Y-miniplex+ (Eight reduced-size Y-STR loci of Yfiler™)
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ABO genotyping
- Rapid direct PCR for ABO blood typing
- ABO blood typing for degraded DNA
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PCR product purification
- Using the QIAquick® PCR Purification Kit
- Using the ExoSAP-IT® for PCR Product Clean-Up
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